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Recalcitrance of the lignocellulose – focus on lignin |
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In this PhD project, novel genes in lignin polymerisation will be identified using two complementary biological systems – one simplified (in vitro cell culture) and one integrated (whole hybrid aspen trees). The overall research strategy resides in using first the simplified system to rapidly and specifically identify and characterise lignin polymerisation candidate genes by combining bioinformatic and proteomic approaches. The most interesting candidate genes will then be functionally demonstrated in the in vitro cell culture using stable transgenic cell lines for overexpression and RNAi. The resulting best candidate genes will then be similarly functionally characterised in hybrid aspen by producing stable transgenic poplar trees for overexpression and RNAi. In each case, the impact on lignification will be estimated using both microscopical and biochemical (wood chemistry, saccharification) techniques.
Supervisor: Edouard Pesquet (
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